Anaerobic Bacteriology

Anaerobic Bacteria

June 2012

It’s important to recognize that anaerobic bacteria are part of the human micro bio.  They are normal flora of the skin, gastrointestinal tract, oropharynx, and female genitourinary/gynecologic tracts.  They play important roles in normal physiology.  Generally they are of low pathogenicitiy except when they occur in “the wrong place at the wrong time” such as a break in normal host barriers such as rupture of the intestinal mucosal barrier.  Anaerobes grow in no or low oxygen concentrations in areas where there are low oxidation reduction potentials in the local environment.

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Anaerobic Bacteria

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions