Anaerobic Bacteriology
Objectives
June 2012
The objectives for this session are the selection of appropriate specimens and methods of collection and transport for anaerobic culture, understanding of current methods for culture and identification of anaerobic bacteria, and the indications and methods for antimicrobial susceptibility testing of anaerobes.
Objectives |
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- Introduction
- Objectives
- Anaerobic Bacteria
- Principal Anaerobic Pathogens
- Specimen Selection: Avoid Contamination With Normal Flora
- Inappropriate Specimens
- Collection and Transport
- Anaerobic Transport Vials
- Anaerobic Transporters
- Anaerobic Culture Media
- Primary Culture CO2 Holding Jar with Flow Meter
- Incubation
- Anaerobic Jars Set Up Using the Anaero-Pack
- Anaerobic Glove Box
- Anaerobe Culture Triage
- Anaerobe Culture Triage (cont.)
- Identification
- Colonial Morphology
- Pigmented Colonies
- Rapid Identification Using Gram Stain
- Identification
- 16S Ribosomal RNA Sequence of Bacteroides fragilis
- Antimicrobial Susceptibility Testing
- AST Methods
- Illustration of the Components of the E-test Method
- Susceptibility Testing by E-test
- Anaerobe Antimicrobial Panels
- References
- Special Thanks
- Questions
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