Dengue Fever

Laboratory Confirmation of Dengue

May 2012

The IgG ELISA is also available as a confirmatory test, if multiple specimens, collected during the course of disease, are used.

This test uses the same antigens as does the MAC-ELISA, the protein E membrane glycoprotein.

The IgG ELISA can differentiate primary or secondary infections, however you must have both acute and convalescent serum specimens. A negative IgG followed by a positive IgG is indicative of a primary infection. A 4-fold rise between acute and convalescent specimens is indicative of a secondary dengue infection. This assay cannot be used to serotype dengue infections.

Commercial tests are available; but they are not FDA-approved at this time.

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Laboratory Confirmation of Dengue

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• Introduction
• Overview
• Dengue Virus
• Structure of Dengue Virus
• History
• History
• Epidemiology of Dengue
• Vectors of Dengue
• Vectors of Dengue
• Life Cycle of Dengue Virus
• Pathogenesis of Dengue
• Classification of Dengue Disease
• Antibody-Dependent Enhancement (ADE)
• Symptoms of Dengue
• Severe Dengue
• Treatment of Dengue
• Diagnosis of Dengue
• Diagnostic Markers for Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Rapid Tests
• Laboratory Confirmation of Dengue
• Future Considerations
• Why Are We Concerned About Dengue?
• Key West, Florida 2009–2010
• Key West, Florida 2009–2010
• Why Re-emergence of Dengue in Florida?
• Summary
• Summary
• Summary
• Summary
• References
• Questions