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Dengue Fever


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Laboratory Confirmation of Dengue

Slide 19

May 2012

Viral cell culture for the dengue virus is performed using the C6/36 cell line of Aedes albopictus for growth – however, this cell line is very unlikely to be available in clinical laboratories.

Viral cell culture is confirmatory, and identifies serotypes via immunofluorescence with monoclonal antibody.

An acute serum sample collected within the first 5 days of symptoms is required for best yield of virus. Growth of dengue virus does not differentiate between primary and secondary infections.

Viral culture is generally performed only at research laboratories or certain state department of health laboratories. Cell culture facilities are required, and testing will usually take at least a week before results are available.

Laboratory Confirmation of Dengue


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