Dengue Fever

Laboratory Confirmation of Dengue

May 2012

Viral cell culture for the dengue virus is performed using the C6/36 cell line of Aedes albopictus for growth – however, this cell line is very unlikely to be available in clinical laboratories.

Viral cell culture is confirmatory, and identifies serotypes via immunofluorescence with monoclonal antibody.

An acute serum sample collected within the first 5 days of symptoms is required for best yield of virus. Growth of dengue virus does not differentiate between primary and secondary infections.

Viral culture is generally performed only at research laboratories or certain state department of health laboratories. Cell culture facilities are required, and testing will usually take at least a week before results are available.

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Laboratory Confirmation of Dengue

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• Introduction
• Overview
• Dengue Virus
• Structure of Dengue Virus
• History
• History
• Epidemiology of Dengue
• Vectors of Dengue
• Vectors of Dengue
• Life Cycle of Dengue Virus
• Pathogenesis of Dengue
• Classification of Dengue Disease
• Antibody-Dependent Enhancement (ADE)
• Symptoms of Dengue
• Severe Dengue
• Treatment of Dengue
• Diagnosis of Dengue
• Diagnostic Markers for Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Laboratory Confirmation of Dengue
• Rapid Tests
• Laboratory Confirmation of Dengue
• Future Considerations
• Why Are We Concerned About Dengue?
• Key West, Florida 2009–2010
• Key West, Florida 2009–2010
• Why Re-emergence of Dengue in Florida?
• Summary
• Summary
• Summary
• Summary
• References
• Questions