Viral Culture

Introduction

April 2012

Welcome to Mayo Medical Laboratories' Hot Topics. These presentations provide short discussions of current topics and may be helpful to you in your practice.

Our presenter for this program is Dr. Bobbie Pritt, Director of the Clinical Virology and Parasitology Laboratory in the Department of Laboratory Medicine and Pathology at Mayo Clinic in Rochester, Minnesota. Dr. Pritt discusses the utility of viral culture — when it is of use and when other tests are suggested for viral identification. Thank you, Dr. Pritt.

Thank you, Sharon, for that introduction. Welcome to a hot topic on Viral Culture: Uses and Pitfalls.

Introduction

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Jump to section:

• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions