Viral Culture

Summary

April 2012

So in summary, although viral culture is considered a “gold standard,” it is rarely the test of choice. This is due to multiple reasons, including the long turnaround time, the limited number of viruses that will grow in routine culture, unlike bacterial culture which will support the growth of numerous common bacteria and the fact that many viruses don’t grow well or grow very slowly in culture. Therefore, I encourage physicians to be familiar with the different viruses associated with various clinical presentations so that the appropriate tests can be ordered.

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• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions