Viral Culture

Suggested Tests for Viral Identification

April 2012

I’d like to now say a word about some other types of specimens. Specifically, bone marrow, blood, and lymph node are specimens from which viruses are rarely identified by culture, and PCR is almost always the test of choice for viral identification. This may include quantitative PCR on whole blood or plasma for viruses such as EBV, CMV, BKV, HHV-6, and adenovirus. Urine is another specimen that rarely yields virus in culture and PCR is typically the test of choice. It is used for detection of BK virus, adenovirus, CMV, and other viruses. In some situations, culture might also be used for detection of mumps virus and adenovirus. And of course, in all of these settings, tests for other organisms such as bacteria and fungi should be considered as appropriate.

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Suggested Tests for Viral Identification

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Jump to section:

• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions