Viral Culture

Suggested Tests for Viral Identification

April 2012

Some physicians will make the point that culture is useful when novel or emerging viruses are suspected such as in samples from patients with: avian influenza, SARS, small pox, monkey pox, emerging pathogens, or even viral agents of bioterrorism. In these settings, however, viral culture in the routine clinical laboratory would not be recommended due to the risk of infection to laboratory personnel. Therefore, the physician should notify the laboratory if one of these agents is suspected so that the specimen can be sent to a specialized laboratory such as the CDC or other public health lab.

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Suggested Tests for Viral Identification

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Jump to section:

• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions