Viral Culture

Best Tests for Identification of Cultivable Viruses

April 2012

Given these limitations of culture, it is not surprising that it has been replaced in most instances by more rapid, sensitive, and specific tests. This chart here shows the viruses that are commonly sought in patient specimens ant the optimal method for detecting them in the laboratory. Notice that the primary test for almost all viruses listed is PCR.

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Best Tests for Identification of Viruses

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Jump to section:

• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions