Viral Culture
Uses and Pitfalls
CMV Shell Vial Results
April 2012
This next slide shows the coverslips after they have been removed from the shell vials and mounted onto slides. The cell sheet on the coverslip is stained with a monoclonal antibody for a specific virus and then the coverslip is examined under a fluorescent microscope. The image to the right shows a positive result for cytomegalovirus with classic green nuclear staining.
CMV Shell Vial Results |
Jump to section:
- Introduction
- Viral Culture
- Tube Culture
- Multiple Roller Racks Containing Tube Cultures
- Technologist Examining the Cell Monolayers
- Special Stage Attachment
- Negative MRC-5 Cell sheet (human diploid fibroblasts)
- MRC-5 Cell sheet with CMV cytopathic effect (CPE)
- Shell Vial Culture
- Shell Vial Culture (continued)
- CMV Shell Vial Results
- Pitfalls of Culture
- Viruses That Grow In Culture and Incubation Time
- Viruses That Do NOT Grow in Routine Cell Culture
- Best Tests for Identification of Cultivable Viruses
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Summary
- Questions
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