Viral Culture

Shell Vial Culture (continued)

April 2012

Then the tube undergoes centrifugation, followed by a short incubation time which can be as little as 24 hours. This is a marked improvement in turn-around-time for viruses such as CMV which can take up to 14 days to grow in traditional tube culture. Finally, the coverslip is removed and stained with specific monoclonal antibodies for a certain virus. There are also some systems available where a color change indicates the presence of a virus. The advantage of this method is that specific viruses can be isolated in a much shorter period of time than is possible with traditional tube culture. Unfortunately, the shell vials are usually set up so that only 1 virus can be identified per tube.

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Shell Vial Culture (continued)

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Jump to section:

• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions