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Viral Culture

Uses and Pitfalls

Shell Vial Culture (continued)

Slide 10

April 2012

Then the tube undergoes centrifugation, followed by a short incubation time which can be as little as 24 hours. This is a marked improvement in turn-around-time for viruses such as CMV which can take up to 14 days to grow in traditional tube culture. Finally, the coverslip is removed and stained with specific monoclonal antibodies for a certain virus. There are also some systems available where a color change indicates the presence of a virus. The advantage of this method is that specific viruses can be isolated in a much shorter period of time than is possible with traditional tube culture. Unfortunately, the shell vials are usually set up so that only 1 virus can be identified per tube.

Shell Vial Culture (continued)


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