Viral Culture
Uses and Pitfalls
MRC-5 Cell sheet with CMV cytopathic effect (CPE)
April 2012
When the cell sheet is infected, as shown on this next slide with cytomegalovirus, the cell sheet shows damage or CPE that is characteristic for the infecting virus. The CPE is noted here by the 2 black arrows. Our technologists are highly trained so that they can recognize the different types of CPE caused by each virus.
MRC-5 Cell sheet with CMV CPE |
Jump to section:
- Introduction
- Viral Culture
- Tube Culture
- Multiple Roller Racks Containing Tube Cultures
- Technologist Examining the Cell Monolayers
- Special Stage Attachment
- Negative MRC-5 Cell sheet (human diploid fibroblasts)
- MRC-5 Cell sheet with CMV cytopathic effect (CPE)
- Shell Vial Culture
- Shell Vial Culture (continued)
- CMV Shell Vial Results
- Pitfalls of Culture
- Viruses That Grow In Culture and Incubation Time
- Viruses That Do NOT Grow in Routine Cell Culture
- Best Tests for Identification of Cultivable Viruses
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Suggested Tests for Viral Identification
- Summary
- Questions
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