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Viral Culture

Uses and Pitfalls

Tube Culture

Slide 3

April 2012

Traditional tube culture involves the use of a tube containing a monolayer of living cells. The monolayer is infected by virus that is present in the patient specimen and the tubes are then incubated on a roller rack such as that shown here on the right to ensure that cells are bathed in media to keep them alive. The cell monolayers are then periodically observed for damage that is consistent with viral infection. This is referred to as cytopathic effect or CPE.

Tube Culture


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