Viral Culture

Viral Culture

April 2012

I’d like to begin with a brief overview of viral culture. As many of you probably know, it is a traditional microbiology method that was previously considered to be the “gold standard” for viral diagnosis although this is now changing with wider use of molecular diagnostic tools. Viral culture uses methods such as tube culture and shell vial culture and it is more sensitive than rapid antigen detection methods such as the ubiquitous “card tests” for influenza and RSV.

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Viral Culture

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• Introduction
• Viral Culture
• Tube Culture
• Multiple Roller Racks Containing Tube Cultures
• Technologist Examining the Cell Monolayers
• Special Stage Attachment
• Negative MRC-5 Cell sheet (human diploid fibroblasts)
• MRC-5 Cell sheet with CMV cytopathic effect (CPE)
• Shell Vial Culture
• Shell Vial Culture (continued)
• CMV Shell Vial Results
• Pitfalls of Culture
• Viruses That Grow In Culture and Incubation Time
• Viruses That Do NOT Grow in Routine Cell Culture
• Best Tests for Identification of Cultivable Viruses
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Suggested Tests for Viral Identification
• Summary
• Questions