Introduction to Clinical Mycology

Safety

March 2012

Safety is a big item in the hospital setting these days and it is also an item in the laboratory where you need to concentrate. Use common sense when you work. Consider all specimens to be infectious and potentially pathogenic. Consider all fungi to be pathogenic, and you work with anything like that inside of a biological safety cabinet particularly just the molds, so you don’t infect someone within the laboratory. Because you never know in your own clinical laboratory who is immunosuppressed and who is not. And, if you are careless and you end up contaminating the laboratory, someone in there could become ill and, if they are highly immunosuppressed, they could become very sick and there have been patients who have died from a laboratory-acquired infection.

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
• Filamentous Fungi
• Filamentous Fungi
• Filamentous Fungi
• Rapid Methods
• Laboratory Diagnosis of Fungal Infections
• Clinical Microbiology Today
• Patient Care
• Sharpen Observation Skills
• Resources - Laboratories
• Communication
• Volunteer
• Safety
• Safety (Continued)
• Working Safely
• Question Things
• Guiding Principles
• Guiding Principles for Professionals
• Summary
• Questions