Introduction to Clinical Mycology

Wet Mount

March 2012

Then you look at it underneath the microscope and, if you can recognize the spores, you can make an identification. If you don’t recognize the spores or how they are arranged, then you have to figure out what the organism is if you are going to base the identification on a morphologic features. And, in most cases, this means trying to go to a key or look in through some books until you find the photograph that matches what you are going to try and identify and which you have in your culture. Today, with nucleic acid sequencing, it is much easier to identify organisms without having to spend all of the time looking at the morphologic features, but it is a costly process.

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
• Filamentous Fungi
• Filamentous Fungi
• Filamentous Fungi
• Rapid Methods
• Laboratory Diagnosis of Fungal Infections
• Clinical Microbiology Today
• Patient Care
• Sharpen Observation Skills
• Resources - Laboratories
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