Introduction to Clinical Mycology

Placement of Coverslip Over Agar

March 2012

And then, you place a cover slip on that. Then you can take something like a pencil eraser and push down on that and allow that to disperse the supporting agar and the organism on there so that you can see it better. There is one thing you will learn very quickly and that is that when you put too much organism on the slide you put the coverslip on there and you push down with the pencil eraser, that piece of agar will shoot across the table. What you have done is contaminated your work site and you have lost the specimen that you were looking for. So pick a moderate amount before you put it underneath that coverslip.

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
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