Introduction to Clinical Mycology

Stain Used for Microscopic Preparations

March 2012

Lactophenol cotton blue or lactophenol aniline blue are 2 dyes that are used commonly. Lactofuchsin is another dye that is not used very often. It stains the organisms red. Lactophenol cotton blue and aniline blue will stain it blue.

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Stain for Microscopic Preparations

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
• Filamentous Fungi
• Filamentous Fungi
• Filamentous Fungi
• Rapid Methods
• Laboratory Diagnosis of Fungal Infections
• Clinical Microbiology Today
• Patient Care
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