Introduction to Clinical Mycology

Wet Mount

March 2012

In terms of how to look at a mold and try to make an identification, there are several ways to do that. One is to make what is called a simple wet mount, where you open up the culture dish and you take a wire that is bent at a right angle, it is sterile wire that you flamed, and you take a small part of the supporting agar along with the culture and you place it on the slide that contains a dye that we use for staining fungi.

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
• Filamentous Fungi
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