Introduction to Clinical Mycology

Introduction to Clinical Mycology

March 2012

This is the introduction to clinical mycology, the final presentation in a series of 4 in clinical mycology. Part 1 deals with the diagnosis, classification, and general features of the fungi and fungal infections. Part 2 dealt with the basic structures of molds and yeasts and a brief introduction to culturing. Part 3 presents specific information on the culturing and incubation of cultures for the optimal recovery. In part 4, we will present methods for identification of fungi, primarily molds, and some helpful hints for working in the clinical laboratory, which we will cover today.

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Introduction to Clinical Mycology

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• Introduction
• Introduction to Clinical Mycology
• General Terms Used in Clinical Mycology
• Typical Mold Colonies
• Identification of Molds
• Wet Mount
• Stain Used for Microscopic Preparations
• Placement of Supporting Agar and Organism on Slide
• Placement of Coverslip Over Agar
• Wet Mount
• Scotch Tape Preparation
• Conidia Still Attached and Characteristically Arranged As Produced
• Microslide Culture
• Slide Culture - Simple Humidity Chamber
• Inoculation of Agar Plugs
• Placement of Coverslips Over Agar Plugs
• Removal and Placement of Coverslip Onto Slide
• Cheap Way to Make a Microslide Culture
• Filamentous Fungi
• Filamentous Fungi
• Filamentous Fungi
• Rapid Methods
• Laboratory Diagnosis of Fungal Infections
• Clinical Microbiology Today
• Patient Care
• Sharpen Observation Skills
• Resources - Laboratories
• Communication
• Volunteer
• Safety
• Safety (Continued)
• Working Safely
• Question Things
• Guiding Principles
• Guiding Principles for Professionals
• Summary
• Questions