HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma

P16 Immunohistochemistry

March 2012

Most studies have found a strong correlation between p16 immunoreactivity and HPV positivity by PCR or ISH. In other words, p16 has a high level of sensitivity.

On the other hand, p16 can be overexpressed even in the absence of HPV DNA or E6/E7 transcripts. For this reason the specificity remains a weakness.

Naturally, p16 interpretation is by way of light microscopy so the histologic correlation goes without saying.

Lastly, not all but a fair number of immunohistochemistry laboratories offer p16 immunostaining.

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P16 Immunohistochemistry

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• Objectives
• Outline
• Oropharyngeal Squamous Cell Carcinoma in Review
• Oropharynx Anatomy
• Epidemiology of OPSCC — Demographics
• Epidemiology of OPSCC — Incidence
• Management Strategies in OPSCC
• The Role of HPV and p16 in Oropharyngeal Squamous Cell Carcinoma
• Human Papillomavirus (HPV)
• HPV in OPSCC Oncogenesis — Genome
• HPV in OPSCC Oncogenesis — Pathways
• HPV in OPSCC Oncogenesis — Detection
• Indications for HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Prognostic
• Prognostic
• Prognostic
• Diagnostic
• Interpretation of HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Interpret the Presence or Absence of HPV
• Don't Jump the Gun
• Polymerase Chain Reaction (PCR)
• DNA In Situ Hybridization
• E6/E7 Messenger Ribonucleic Acid (mRNA)
• P16 Immunohistochemistry
• HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma in Review
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