HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma

E6/E7 Messenger Ribonucleic Acid (mRNA)

March 2012

Detecting E6/E7 mRNA transcripts provides exquisite sensitivity and specificity. Moreover, this assay affords tremendous insight into the expression status of oncogenes E6 and E7. The presence of E6 and E7 mRNA transcripts, for all intents and purposes, represents biologically active HPV DNA, regardless of localization.

Relatively new systems allow for examination with the light microscope which grants obvious advantages.

Unfortunately, this can be a cumbersome test to perform and is not available in the vast majority of laboratories on a clinical basis.

< Previous Slide

E6/E7 mRNA

Next Slide >

 

Jump to section:

• Objectives
• Outline
• Oropharyngeal Squamous Cell Carcinoma in Review
• Oropharynx Anatomy
• Epidemiology of OPSCC — Demographics
• Epidemiology of OPSCC — Incidence
• Management Strategies in OPSCC
• The Role of HPV and p16 in Oropharyngeal Squamous Cell Carcinoma
• Human Papillomavirus (HPV)
• HPV in OPSCC Oncogenesis — Genome
• HPV in OPSCC Oncogenesis — Pathways
• HPV in OPSCC Oncogenesis — Detection
• Indications for HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Prognostic
• Prognostic
• Prognostic
• Diagnostic
• Interpretation of HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Interpret the Presence or Absence of HPV
• Don't Jump the Gun
• Polymerase Chain Reaction (PCR)
• DNA In Situ Hybridization
• E6/E7 Messenger Ribonucleic Acid (mRNA)
• P16 Immunohistochemistry
• HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma in Review
• Review
• Contact Information
• References
• References
• Questions