HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma

Prognostic

March 2012

As mentioned previously, HPV DNA in situ hybridization can also reveal a fully integrated pattern. This is recognizable by punctate nuclear signals as you can appreciate in this micrograph. Although several studies have used HPV in situ hybridization as a means of classifying tumors as HPV-positive or negative, the pattern of positivity is rarely addressed. That is, little effort has been made to distinguish episomal from fully integrated from fully mixed patterns of positivity. It has been shown in squamous cell carcinoma cell lines that E6 and E7 can be expressed in the setting of both episomal and integrated HPV DNA. Currently, it is unclear if these patterns fall out into different prognostic or therapeutic categories.

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• Objectives
• Outline
• Oropharyngeal Squamous Cell Carcinoma in Review
• Oropharynx Anatomy
• Epidemiology of OPSCC — Demographics
• Epidemiology of OPSCC — Incidence
• Management Strategies in OPSCC
• The Role of HPV and p16 in Oropharyngeal Squamous Cell Carcinoma
• Human Papillomavirus (HPV)
• HPV in OPSCC Oncogenesis — Genome
• HPV in OPSCC Oncogenesis — Pathways
• HPV in OPSCC Oncogenesis — Detection
• Indications for HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Prognostic
• Prognostic
• Prognostic
• Diagnostic
• Interpretation of HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma
• Interpret the Presence or Absence of HPV
• Don't Jump the Gun
• Polymerase Chain Reaction (PCR)
• DNA In Situ Hybridization
• E6/E7 Messenger Ribonucleic Acid (mRNA)
• P16 Immunohistochemistry
• HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma in Review
• Review
• Contact Information
• References
• References
• Questions