Introduction to Clinical Mycology

Sealing of Culture Dish to Prevent Contamination

February 2012

If you happen to have a culture that grows up a significant pathogen, or an organism that grows a lot of volume of an organism that is going to contaminate your incubator, you need to seal the plate with tape if you plan to keep it for any length of time. If it is something that you have already identified and it looks like it’s going to be a problem, you can simply just autoclave it. But if you are going to keep it for a significant period of time, make sure you tape it up with oxygen-permeable tape.

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Sealing of Culture Dish to Prevent Contamination

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions