Introduction to Clinical Mycology

Culture Incubation

February 2012

Terms of incubation of cultures–you can incubate the cultures in any incubators, in any way you like. At Mayo Clinic what we use are cafeteria trays. And you notice that all of the culture dishes are placed in bags and sealed. There is a reason for this, it prevents dehydration, but it also allows us to work safely with the cultures, that we can read those plates through the bags without having to take them out.

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Culture Incubation

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
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