Introduction to Clinical Mycology

Use of Tubes for Culture: Advantages and Disadvantages

February 2012

Use of tubes for cultures. The advantages and disadvantages of those, well, culture tubes certainly you have less dehydration because generally, the lids are kept tight. And, you have the lack of isolation of colonies in there because when you inoculate the tube, the substance all runs to the bottom, or the butt, of the slant and everything grows in one place. If the caps are tightened, no air is going to be able to get in, no oxygen will get in there and the cultures will become nonviable, so the lids need to be left slightly ajar. Culture tubes are certainly safe to handle, and in most instances are safer to handle because most people do tighten the caps so nothing can get out, and you can’t do that. It is very difficult to make a mount from down inside one of those tubes; it can be done, but it is not easy. And, certainly, a culture plate is easier for that to happen.

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
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