Introduction to Clinical Mycology

Use of Culture Dishes: Advantages and Disadvantages

February 2012

You notice that what we have shown before have been culture dishes and there certainly are advantages and disadvantages to using these. In laboratories with little experience, this is probably not a very good idea; it is a risky situation to use culture plates. But in a laboratory that does a moderate to great volume of fungal cultures, these culture plates allow you to have a large surface area for the isolation of colonies. It provides good aeration of colonies. It is certainly easier to make a microscopic mount by just simply opening up a culture dish and making the mount. One of the problems is it is easy to get these plates contaminated. An open system is less safe to handle in the laboratory. And culture dishes are apt to dry out unless you use deep pour plates. Deep pour plates are meant to be like 40 mL of culture medium or more.

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions