Introduction to Clinical Mycology

Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment

February 2012

This happens to be Blastomyces that was recovered from a clinical specimen. And if you look at the right-hand side of the plate you can see bacterial colonies along there along with some of the fungal colonies, and as it gets further across to the left-hand side of the plate, there are just fungal colonies. This allowed for the organism to be recovered, whereas, it would have been missed probably or not grown well at all on a plate that didn’t have the ammonium hydroxide added to it.

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Culture After Ammonium Hydroxide Treatment

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
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