Introduction to Clinical Mycology

Culture of Coccidioides immitis on a Medium with Cycloheximide

February 2012

This is Coccidioides. These are colonies of Coccidioides except for the one on the left-hand side about 9 o’clock that is bright white. All of the other colonies are Coccidioides immitis on a culture plate. They came from a contaminated specimen and cycloheximide allowed those colonies that were slower growing to be able to grow with out being inhibited by a rapidly growing mold.

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Culture of Coccidioides immitis

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions