Introduction to Clinical Mycology

Aspergillus fumigatus

February 2012

This is the example of Aspergillus fumigatus and on the plate on the right-hand side; the culture dish shows no growth. This is the culture plate that contains cycloheximide. If we were looking for that organism, causing aspergillosis in a patient, and we only used that particular medium, we would miss it. It would not grow and we would never see it and so we would miss the diagnosis. So the 2 plates that lack cycloheximide you can see grow Aspergillus fumigatus very well.

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Aspergillus fumigatus

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions