Introduction to Clinical Mycology

Enhancement Supplements for Fungal Recovery Media

February 2012

We have shown in the past that we need certain compounds added to the culture media to allow us to recover the organism that we want to find. And the reason for that is there are bacterial overgrowths in many instances, because the specimens that we get for cultures we get are often contaminated. Gentamicin, chloramphenicol are 2 antibiotics that can be used in combination to inhibit many of the bacteria that will allow the fungi to grow through fungal media. Ciprofloxacin can be added to culture media and that is used to try to inhibit the growth in things like pseudomonas. Cycloheximide, another name is actidione, is a compound that we will see in just a moment here that is used for preventing the rapid overgrowth by some molds of slower growing molds. And then there are certain instances where we have used media that contain sheep blood enrichment because the organisms may require a bit more enrichment before they are able to be recovered.

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Supplements for Fungal Recovery Media

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions