Introduction to Clinical Mycology

Introduction to Clinical Mycology

February 2012

This is another series in an introduction to clinical mycology, the third in a series of 4 presentations. Part 1 was diagnosis of classification and general features of the fungi and fungal infections. Part 2 was the basic structures of molds and yeasts and a brief introduction to culture. Part 3 presents specific information on the culturing and the incubation of cultures for the optimal recovery of fungi, and we will cover this today.

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Introduction to Clinical Mycology

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• Introduction
• Introduction to Clinical Mycology
• Culture Variation of Cryptococcus neoformans–Medium Dependent
• Enhancement Supplements for Fungal Recovery Media
• Use of Cycloheximide
• Cycloheximide: Fungi Inhibited
• Aspergillus fumigatus
• Effect of Cycloheximide–Aspergillus niger
• Culture of Coccidioides immitis on a Medium with Cycloheximide
• Treatment of Contaminated Specimens
• Typical Overgrowth of Culture Plate
• Culture of Blastomyces dermatitidis After Ammonium Hydroxide Treatment
• Use of Culture Dishes: Advantages and Disadvantages
• Use of Culture Dishes: Dehydration of Media
• Use of Tubes for Culture: Advantages and Disadvantages
• Use of Culture Tubes
• Incubation of Cultures
• Culture Incubation
• Culture Examination
• Sealing of Culture Dish to Prevent Contamination
• Mitey Big Problem
• Introduction to Clinical Mycology
• Questions