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Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry


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Misidentification by Bruker Biotyper3

Slide 26

February 2012

There were some limitations. Four of 10 Streptococcus mitis species group isolates were misidentified as Streptococcus pneumoniae using extraction. All 10 Streptococcus pneumoniae were correctly identified. This finding was previously reported. The difficulty in distinguishing between closely related species may be due to the similar composition of ribosomal proteins, the main target for matrix-assisted laser desorption ionization time of flight mass spectrometry. Two Enterococcus casseliflavus/gallinarum were misidentified as Enterococcus phoeniculicola using extraction and one by direct colony testing. Enterococcus phoeniculicola has been isolated from birds and it is not known to cause human disease. This misidentification was previously reported. In one report, correct identification of Enterococcus casseliflavus was reached when the library was updated. We tested one of our isolates using preparatory extraction after implementing an update to our library and also confirmed Enterococcus casseliflavus.

Misidentification by Bruker Biotyper3


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