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Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry


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Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry3

Slide 23

February 2012

Isolates were cultured overnight on 5% sheep blood agar at 35ºC, except Granulicatella and Abiotrophia species, which were cultured on chocolate agar. Results were compared to phenotypic testing and partial 16S ribosomal RNA gene sequencing. Seven isolates were excluded as they were not present in the Bruker Biotyper database, leaving 298 isolates. Discrepant results were resolved by additional biochemical analysis and/or partial 16S ribosomal RNA gene sequencing. Colonies were tested directly and subjected to extraction.

Identification of Gram-Positive Cocci3


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