Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³

February 2012

Isolates were cultured overnight on 5% sheep blood agar at 35ºC, except Granulicatella and Abiotrophia species, which were cultured on chocolate agar. Results were compared to phenotypic testing and partial 16S ribosomal RNA gene sequencing. Seven isolates were excluded as they were not present in the Bruker Biotyper database, leaving 298 isolates. Discrepant results were resolved by additional biochemical analysis and/or partial 16S ribosomal RNA gene sequencing. Colonies were tested directly and subjected to extraction.

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Identification of Gram-Positive Cocci3

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Jump to section:

• Introduction
• Objectives
• How Does Mass Spectrometry Work?
• Types of Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Time of Flight
• Mass Spectrum Generated Compared with Library (Database)
• Commercial MALDI TOF Systems for Microbial Identification
• MALDI TOF Mass Spectrometry Preparatory Extraction*
• Bacterial Identification by Mass Spectrometry
• Bacterial Identification by MALDI TOF Mass Spectrometry¹
• Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Gram-Positive Cocci³
• Gram-Positive Cocci³
• Misidentification by Bruker Biotyper³
• MALDI TOF Mass Spectrometry: Strengths
• MALDI TOF Mass Spectrometry: Limitations
• Bacteriology Laboratory Workflow Today
• Workflow with Mass Spectrometry
• References
• Acknowledgments
• Questions