Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²

February 2012

We have recently published 2 studies evaluating matrix-assisted laser desorption ionization time of flight mass spectrometry for bacterial identification. The first compared the Bruker mass spectrometry system to the BD Phoenix Automated Microbiology System for identification of Gram-negative bacilli. A Microflex LT mass spectrometer was used in conjunction with Bruker Biotyper MALDI Automation Control software version 2.0 and a Biotyper library containing 3740 reference spectra. The system generates a score; criteria used to attain species- and genus-level identifications are shown. A score of ³2.0 indicates acceptable species identification (assuming that there is at least a 10% score difference between the top match and different genera or species with closely related spectra). A score of 1.7 to 1.999 indicates acceptable genus identification. If the score is <1.7, the system does not reliably identify the organism. These score cutoffs were specified by Bruker Daltonics. Discrepant results were resolved by conventional biochemicals and/or 16S ribosomal RNA gene or gyrB sequencing.

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Jump to section:

• Introduction
• Objectives
• How Does Mass Spectrometry Work?
• Types of Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Time of Flight
• Mass Spectrum Generated Compared with Library (Database)
• Commercial MALDI TOF Systems for Microbial Identification
• MALDI TOF Mass Spectrometry Preparatory Extraction*
• Bacterial Identification by Mass Spectrometry
• Bacterial Identification by MALDI TOF Mass Spectrometry¹
• Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Gram-Positive Cocci³
• Gram-Positive Cocci³
• Misidentification by Bruker Biotyper³
• MALDI TOF Mass Spectrometry: Strengths
• MALDI TOF Mass Spectrometry: Limitations
• Bacteriology Laboratory Workflow Today
• Workflow with Mass Spectrometry
• References
• Acknowledgments
• Questions