Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Mass Spectrum Generated Compared with Library (Database)

February 2012

The mass profile is used as a fingerprint or mass spectrum (as shown here) to compare with those of well-characterized organisms in a database. The spectrum typically includes genus- and species-specific peaks, so that with a comprehensive library of spectra, the genus and often the species of the organism may be determined using bioinformatics.

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Mass Spectrum Compared with Library

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Jump to section:

• Introduction
• Objectives
• How Does Mass Spectrometry Work?
• Types of Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Time of Flight
• Mass Spectrum Generated Compared with Library (Database)
• Commercial MALDI TOF Systems for Microbial Identification
• MALDI TOF Mass Spectrometry Preparatory Extraction*
• Bacterial Identification by Mass Spectrometry
• Bacterial Identification by MALDI TOF Mass Spectrometry¹
• Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Gram-Positive Cocci³
• Gram-Positive Cocci³
• Misidentification by Bruker Biotyper³
• MALDI TOF Mass Spectrometry: Strengths
• MALDI TOF Mass Spectrometry: Limitations
• Bacteriology Laboratory Workflow Today
• Workflow with Mass Spectrometry
• References
• Acknowledgments
• Questions