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Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry



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Time of Flight

Slide 10

February 2012

Next the mass of the ions is analyzed. This is accomplished using a flight tube, the lighter ions traveling faster and, therefore, being detected earlier than the heavier ions. In the described method, particles are typically singly charged. The net result is generation of a mass spectrum in which the mass-to-charge is plotted against signal intensity. Only highly abundant proteins that are of low mass and ionize readily are detected. These are typically ribosomal proteins, although the specific nature of the analyzed proteins is not part of the analysis.

Time of Flight

 


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