Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Matrix-Assisted Laser Desorption Ionization

February 2012

The matrix absorbs energy from the laser, releasing it into the sample as heat. This causes the sample to desorb and form singly charged ions, the "desorption and ionization" component of matrix-assisted laser desorption ionization.

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Jump to section:

• Introduction
• Objectives
• How Does Mass Spectrometry Work?
• Types of Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Time of Flight
• Mass Spectrum Generated Compared with Library (Database)
• Commercial MALDI TOF Systems for Microbial Identification
• MALDI TOF Mass Spectrometry Preparatory Extraction*
• Bacterial Identification by Mass Spectrometry
• Bacterial Identification by MALDI TOF Mass Spectrometry¹
• Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Gram-Positive Cocci³
• Gram-Positive Cocci³
• Misidentification by Bruker Biotyper³
• MALDI TOF Mass Spectrometry: Strengths
• MALDI TOF Mass Spectrometry: Limitations
• Bacteriology Laboratory Workflow Today
• Workflow with Mass Spectrometry
• References
• Acknowledgments
• Questions