Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper

February 2012

The target plate is placed into the plate chamber of the mass spectrometer, the plate chamber closed, and analysis performed. A 24-spot target plate can be prepared and analyzed in under an hour. This includes spotting the colonies, adding matrix, and analyzing the spectra, equating to ~2 to 3 min/sample.

< Previous Slide

Bruker Biotyper

Next Slide >

 

Jump to section:

• Introduction
• Objectives
• How Does Mass Spectrometry Work?
• Types of Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
• Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry - Bruker Biotyper
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Matrix-Assisted Laser Desorption Ionization
• Time of Flight
• Mass Spectrum Generated Compared with Library (Database)
• Commercial MALDI TOF Systems for Microbial Identification
• MALDI TOF Mass Spectrometry Preparatory Extraction*
• Bacterial Identification by Mass Spectrometry
• Bacterial Identification by MALDI TOF Mass Spectrometry¹
• Bruker Biotyper MALDI TOF Mass Spectrometry versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Bruker Biotyper MALDI TOF Mass Spectrometer versus BD Phoenix Automated Microbiology System Identification of Gram-Negative Bacilli²
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Identification of Gram-Positive Cocci by Bruker Biotyper Mass Spectrometry³
• Gram-Positive Cocci³
• Gram-Positive Cocci³
• Misidentification by Bruker Biotyper³
• MALDI TOF Mass Spectrometry: Strengths
• MALDI TOF Mass Spectrometry: Limitations
• Bacteriology Laboratory Workflow Today
• Workflow with Mass Spectrometry
• References
• Acknowledgments
• Questions