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Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry



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Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Slide 5

February 2012

I will first go over how matrix-assisted laser desorption ionization time of flight mass spectrometry is practically done in the clinical laboratory, starting from a colony. There are several systems available; I will overview the Bruker Daltonics system, the system with which I have the most experience. A colony may be picked directly from a plate and then smeared onto a target plate. Then, 1 to 2 mcL of a “matrix” consisting, for example, of alpha-cyano-4-hydroxycinnamic acid dissolved in acetonitrile and trifluoroacetic acid, is added and dried on the plate.

Laser Desorption Ionization Time

 


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