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Introduction to Clinical Mycology

Part 2



Microscopic Examination of Clinical Specimens: Detection of Fungi (Continued)

Slide 29

January 2012

In terms in the topic of microscopy that can be used to examine the clinical specimens, bright-field microscopy can be used for things like Gram-stain and so on. It can be used also if you turn the light down or you close the iris diaphragm down you can get some contrast. You can see organisms in clinical specimens. Phase-contrast microscopy is not used so often any more but it does a nice job of being able to allow you to see fungal structures. The Auramine-Rhodamine stain is used in a mycobacteriology laboratory. It can stain organisms like Blastomycetes. The Ziehl-Neelsen or the Kinyoun stain is an acid--fast stain that uses methylene blue as a counter stain. And the organisms may not necessarily stain acid- fast, but you can see them with a counter stain. So you have to look beyond the concentrated acid- fast part of the stain. The Papanicolaou's stain is used in cytology for looking for cancer cells. You can see fungi in the Pap smears, the sputum, and other clinical specimens. And then in the clinical laboratory where the pathology is involved, you notice the Gomori methenamine silver, the H&E, the PAS stain, all those stains there. Some of them are used to detect tissue morphology; others are used to detect fungi. But when you are looking at this section, you have to be thinking about anything, and the fungi are one of the things a pathologist has to be looking for. The microbiology laboratory can be of some help recognizing some of those fungal elements.

Detection of Fungi (Continued)

 


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