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Introduction to Clinical Mycology

Part 2

Calcofluor White

Slide 27

January 2012

Calcofluor white is used as I mentioned as a fluorescent brightener. It is a nonspecific stain that binds with a chitin in the cell wall of the fungi. You have to use certain filter combinations, 440 nanometer filters. They are very costly but they work well. And the calcofluor white needs to be made up ahead of time and kept in a bottle that is dark and is covered with foil because it is light labile. But it is a very useful tool in the clinical laboratory to be able to stain to look at the fungi.

Calcofluor White


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