The Classic Myeloproliferative Neoplasms

JAK2 Exon 12 Sequencing

October 2011

When we looked back at 92 cases at Mayo Clinic that had JAK2 exon 12 sequencing studies done, we found that only 25% were appropriately requested–those that had a negative V617F and had a PV-like CBC with decreased serum erythropoietin. 75% of these 92 requests appeared to be inappropriately ordered since they had no JAK2 V617F testing, or had positive JAK2 V617F results, or had a bone marrow with no morphologic features of a myeloproliferative disorder, or a peripheral blood that had no PV-like CBC with normal serum erythropoietin values.

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JAK2 Exon 12 Sequencing

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• Introduction
• Optimizing Laboratory Testing for Hematologic Disorders Series
• Goals for Today's Presentation
• Example of a Recent MPN Referral
• Why Do We Have Test Utilization Issues?
• Why Do We Have Test Utilization Issues?
• So What Should We Do?
• WHO Classification
• Important Laboratory Assays in the MPNs
• JAK2 V617F Background
• JAK2 V617F Background
• Question #1 — JAK2 V617F
• JAK2 V617F
• JAK2 V617F at Mayo Clinic
• Conclusion #1 — JAK2 V617F
• JAK2 Exon 12 Sequencing Background
• Question #2 — JAK2 Exon 12 Sequencing
• JAK2 Exon 12 Sequencing: When Not to Use
• JAK2 Exon 12 Sequencing
• Conclusion #2 — JAK2 Exon 12 Sequencing
• MPL Exon 10 Sequencing Studies: Background
• Question #3 — MPL Exon 10 Sequencing
• MPL Exon 10 Sequencing: When to Use
• MPL Exon 10 Sequencing: When Not to Use
• MPL Exon 10 Sequencing
• Conclusion #3 — MPL Exon 10 Sequencing
• Roles of Other Assays in Classic MPN
• Summary
• Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
• Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
• Implementing a Utilization Approach
• Questions