Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis
The Role of von Willebrand Factor Multimer Analysis in Diagnosis
VWF Multimers Analysis: Summary (2)
July 2011
Von Willebrand factor multimer analysis should be performed only reflexively after initial screening tests such as von Willebrand factor antigen and activity are found to be reduced or have an abnormal ratio as outlined in the von Willebrand disease profile. There are certainly selected clinical conditions such as in patients with myeloproliferative disorders, hypertrophic obstructive cardiomyopathy or aortic stenosis where multimer analysis may be useful despite normal results of von Willebrand factor assays. However, the cutoffs for von Willebrand factor activity to antigen ratio validated during development of the von Willebrand disease profile have been set to be highly sensitive for detection of a acquired abnormalities of von Willebrand factor.
VWF Multimers Analysis: Summary (2) |
Jump to section:
- Introduction
- Objectives
- Objectives
- VWF Structure and Domains
- VWF: Synthesis and Multimerization
- Synthesis and Catabolism of VWF
- Inactive Platelets With Intact Endothelial Wall
- Activated Platelets: Disrupted Vascular Lumen
- Objectives
- Case History: 23-Year-Old Female
- Prevalence of Bleeding Disorders
- Von Willebrand Disease Testing
- Von Willebrand Disease Profile
- 23-Year-Old Female
- Indication for VWF multimer analysis
- Indication for VWF multimer analysis
- Objectives
- Plasma VWF Multimer Analysis
- Example of von Willebrand Factor Multimer Gel Plate
- Solidfied Gel Template
- Gel Template Plasma Sample Wells
- Electrophoresis Separated von Willebrand Factor Multimers
- Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
- VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
- VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
- Scanning System for Gels
- VWF Multimer Imaging — Near Infrared In-gel Immunostaining
- Autoradiography and Infrared Method Comparison
- VWF Multimer Analysis
- Objectives
- 23-Year-Old Female: Type 1 VWD
- Type 3 VWD
- Type 2A VWD
- Type 2B VWD
- Type 2M VWD
- VWF Multimers Analysis: Summary
- VWF Multimers Analysis: Summary (2)
- References
- Questions?
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