Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis
The Role of von Willebrand Factor Multimer Analysis in Diagnosis
Type 3 VWD
July 2011
In the next case, this patient had markedly reduced von Willebrand factor levels. As a result of such marked reduction, factor VIII activity was also reduced. These data suggest either a severe type 1 or a type 3 von Willebrand disease. Analysis of this patient’s von Willebrand factor multimers essentially demonstrated absent von Willebrand factor multimers. Given the virtual absence of von Willebrand factor multimers, we do not attempt a desmopressin trial. Prevention and management of minor or major hemorrhage for patients with this type of von Willebrand disease would consist of von Willebrand factor concentrates.
Type 3 VWD |
Jump to section:
- Introduction
- Objectives
- Objectives
- VWF Structure and Domains
- VWF: Synthesis and Multimerization
- Synthesis and Catabolism of VWF
- Inactive Platelets With Intact Endothelial Wall
- Activated Platelets: Disrupted Vascular Lumen
- Objectives
- Case History: 23-Year-Old Female
- Prevalence of Bleeding Disorders
- Von Willebrand Disease Testing
- Von Willebrand Disease Profile
- 23-Year-Old Female
- Indication for VWF multimer analysis
- Indication for VWF multimer analysis
- Objectives
- Plasma VWF Multimer Analysis
- Example of von Willebrand Factor Multimer Gel Plate
- Solidfied Gel Template
- Gel Template Plasma Sample Wells
- Electrophoresis Separated von Willebrand Factor Multimers
- Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
- VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
- VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
- Scanning System for Gels
- VWF Multimer Imaging — Near Infrared In-gel Immunostaining
- Autoradiography and Infrared Method Comparison
- VWF Multimer Analysis
- Objectives
- 23-Year-Old Female: Type 1 VWD
- Type 3 VWD
- Type 2A VWD
- Type 2B VWD
- Type 2M VWD
- VWF Multimers Analysis: Summary
- VWF Multimers Analysis: Summary (2)
- References
- Questions?
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