Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis

VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining

July 2011

After the electrophoresis is completed, the gels containing patient von Willebrand factor are subject to multiple incubation and washing steps as shown on the slide. As you can see on the right, our original radioactive method resulted in a processing time of approximately 7 to 9 days. With our developmental efforts, using an infrared-based system, processing time has been reduced to approximately 4 days, and has also eliminated the need for radioactive reagents.

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VWF Multimer Imaging

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Jump to section:

• Introduction
• Objectives
• Objectives
• VWF Structure and Domains
• VWF: Synthesis and Multimerization
• Synthesis and Catabolism of VWF
• Inactive Platelets With Intact Endothelial Wall
• Activated Platelets: Disrupted Vascular Lumen
• Objectives
• Case History: 23-Year-Old Female
• Prevalence of Bleeding Disorders
• Von Willebrand Disease Testing
• Von Willebrand Disease Profile
• 23-Year-Old Female
• Indication for VWF multimer analysis
• Indication for VWF multimer analysis
• Objectives
• Plasma VWF Multimer Analysis
• Example of von Willebrand Factor Multimer Gel Plate
• Solidfied Gel Template
• Gel Template Plasma Sample Wells
• Electrophoresis Separated von Willebrand Factor Multimers
• Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
• VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
• VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
• Scanning System for Gels
• VWF Multimer Imaging — Near Infrared In-gel Immunostaining
• Autoradiography and Infrared Method Comparison
• VWF Multimer Analysis
• Objectives
• 23-Year-Old Female: Type 1 VWD
• Type 3 VWD
• Type 2A VWD
• Type 2B VWD
• Type 2M VWD
• VWF Multimers Analysis: Summary
• VWF Multimers Analysis: Summary (2)
• References
• Questions?