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Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis

The Role of von Willebrand Factor Multimer Analysis in Diagnosis

Plasma VWF Multimer Analysis

Slide 18

July 2011

Broadly considered, the steps of von Willebrand factor multimer analysis consists of denaturation of plasma von Willebrand factor multimer with an anionic detergent, and then subjecting the plasma sample to agarose gel electrophoresis to separate the multimers. Generally, a discontinuous gel system helps in improving resolution, and this resolution will vary with the agarose gel concentration. At this stage, most laboratories electroblot the immobilized von Willebrand factor multimers from the agarose gel onto a membrane for subsequent labeling with an anti- von Willebrand factor antibody which may be radioactive or nonradioactive, chemiluminescent antibodies. At the Special Coagulation Laboratory, Mayo Clinic Rochester, our technique has always consisted of an in-gel analysis system which was originally radioactive, but more recently is an infrared based labeling system, thus avoiding radioactivity. The final step consists of a review of the multimers and interpretation taking into account the von Willebrand factor assay results.

Plasma VWF Multimer Analysis


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