Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis
The Role of von Willebrand Factor Multimer Analysis in Diagnosis
Plasma VWF Multimer Analysis
July 2011
Broadly considered, the steps of von Willebrand factor multimer analysis consists of denaturation of plasma von Willebrand factor multimer with an anionic detergent, and then subjecting the plasma sample to agarose gel electrophoresis to separate the multimers. Generally, a discontinuous gel system helps in improving resolution, and this resolution will vary with the agarose gel concentration. At this stage, most laboratories electroblot the immobilized von Willebrand factor multimers from the agarose gel onto a membrane for subsequent labeling with an anti- von Willebrand factor antibody which may be radioactive or nonradioactive, chemiluminescent antibodies. At the Special Coagulation Laboratory, Mayo Clinic Rochester, our technique has always consisted of an in-gel analysis system which was originally radioactive, but more recently is an infrared based labeling system, thus avoiding radioactivity. The final step consists of a review of the multimers and interpretation taking into account the von Willebrand factor assay results.
Plasma VWF Multimer Analysis |
Jump to section:
- Introduction
- Objectives
- Objectives
- VWF Structure and Domains
- VWF: Synthesis and Multimerization
- Synthesis and Catabolism of VWF
- Inactive Platelets With Intact Endothelial Wall
- Activated Platelets: Disrupted Vascular Lumen
- Objectives
- Case History: 23-Year-Old Female
- Prevalence of Bleeding Disorders
- Von Willebrand Disease Testing
- Von Willebrand Disease Profile
- 23-Year-Old Female
- Indication for VWF multimer analysis
- Indication for VWF multimer analysis
- Objectives
- Plasma VWF Multimer Analysis
- Example of von Willebrand Factor Multimer Gel Plate
- Solidfied Gel Template
- Gel Template Plasma Sample Wells
- Electrophoresis Separated von Willebrand Factor Multimers
- Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
- VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
- VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
- Scanning System for Gels
- VWF Multimer Imaging — Near Infrared In-gel Immunostaining
- Autoradiography and Infrared Method Comparison
- VWF Multimer Analysis
- Objectives
- 23-Year-Old Female: Type 1 VWD
- Type 3 VWD
- Type 2A VWD
- Type 2B VWD
- Type 2M VWD
- VWF Multimers Analysis: Summary
- VWF Multimers Analysis: Summary (2)
- References
- Questions?
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