Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis
The Role of von Willebrand Factor Multimer Analysis in Diagnosis
Indication for VWF multimer analysis
July 2011
In addition to subtyping congenital von Willebrand disease, von Willebrand factor multimer analysis may be useful in certain acquired conditions. Some patients with aortic stenosis, hypertrophic obstructive cardiomyopathy, and in those with left ventricular assist devices experience bleeding, typically gastrointestinal bleeding. In this subset of patients, investigation into the von Willebrand factor multimer structure may provide clues to the cause of bleeding.
Indication for VWF multimer analysis |
Jump to section:
- Introduction
- Objectives
- Objectives
- VWF Structure and Domains
- VWF: Synthesis and Multimerization
- Synthesis and Catabolism of VWF
- Inactive Platelets With Intact Endothelial Wall
- Activated Platelets: Disrupted Vascular Lumen
- Objectives
- Case History: 23-Year-Old Female
- Prevalence of Bleeding Disorders
- Von Willebrand Disease Testing
- Von Willebrand Disease Profile
- 23-Year-Old Female
- Indication for VWF multimer analysis
- Indication for VWF multimer analysis
- Objectives
- Plasma VWF Multimer Analysis
- Example of von Willebrand Factor Multimer Gel Plate
- Solidfied Gel Template
- Gel Template Plasma Sample Wells
- Electrophoresis Separated von Willebrand Factor Multimers
- Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
- VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
- VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
- Scanning System for Gels
- VWF Multimer Imaging — Near Infrared In-gel Immunostaining
- Autoradiography and Infrared Method Comparison
- VWF Multimer Analysis
- Objectives
- 23-Year-Old Female: Type 1 VWD
- Type 3 VWD
- Type 2A VWD
- Type 2B VWD
- Type 2M VWD
- VWF Multimers Analysis: Summary
- VWF Multimers Analysis: Summary (2)
- References
- Questions?
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