Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis
The Role of von Willebrand Factor Multimer Analysis in Diagnosis
Indication for VWF multimer analysis
July 2011
Let us address the question of why von Willebrand factor multimer analysis needs to be performed. As you can see, the main reason to perform von Willebrand factor multimer analysis is to sub-type von Willebrand disease. The main clinically relevant distinction is between type 1 von Willebrand disease and what I like to term the non-type 1 von Willebrand disease, that is the type 2 variants and type 3 von Willebrand disease. This distinction has therapeutic implications. For the type 1 von Willebrand disease, desmopressin or DDAVP, which may be administered by intravenous or intranasal route, may be an option for prevention or treatment of minor hemorrhage. For most type 2 variants and all patients with type 3 von Willebrand disease, von Willebrand factor containing clotting factor concentrates are the treatment of choice.
Indication for VWF multimer analysis |
Jump to section:
- Introduction
- Objectives
- Objectives
- VWF Structure and Domains
- VWF: Synthesis and Multimerization
- Synthesis and Catabolism of VWF
- Inactive Platelets With Intact Endothelial Wall
- Activated Platelets: Disrupted Vascular Lumen
- Objectives
- Case History: 23-Year-Old Female
- Prevalence of Bleeding Disorders
- Von Willebrand Disease Testing
- Von Willebrand Disease Profile
- 23-Year-Old Female
- Indication for VWF multimer analysis
- Indication for VWF multimer analysis
- Objectives
- Plasma VWF Multimer Analysis
- Example of von Willebrand Factor Multimer Gel Plate
- Solidfied Gel Template
- Gel Template Plasma Sample Wells
- Electrophoresis Separated von Willebrand Factor Multimers
- Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
- VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
- VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
- Scanning System for Gels
- VWF Multimer Imaging — Near Infrared In-gel Immunostaining
- Autoradiography and Infrared Method Comparison
- VWF Multimer Analysis
- Objectives
- 23-Year-Old Female: Type 1 VWD
- Type 3 VWD
- Type 2A VWD
- Type 2B VWD
- Type 2M VWD
- VWF Multimers Analysis: Summary
- VWF Multimers Analysis: Summary (2)
- References
- Questions?
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