Von Willebrand Disease (VWD)
Part 4: VWF Multimer Analysis

Synthesis and Catabolism of VWF

July 2011

Upon secretion, the ultra-large von Willebrand factor molecular weight multimer complexes undergo physiologic proteolysis by a mettaloprotease called ADAMTS-13. Thus, circulating plasma von Willebrand factor multimers reflect an equilibrium between secretion of von Willebrand factor containing large and ultra-large multimers and their cleavage into smaller derivatives which when visualized on agarose electrophoresis gels are categorized into low, intermediate and large or high molecular weight multimers.

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Synthesis and Catabolism of VWF

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Jump to section:

• Introduction
• Objectives
• Objectives
• VWF Structure and Domains
• VWF: Synthesis and Multimerization
• Synthesis and Catabolism of VWF
• Inactive Platelets With Intact Endothelial Wall
• Activated Platelets: Disrupted Vascular Lumen
• Objectives
• Case History: 23-Year-Old Female
• Prevalence of Bleeding Disorders
• Von Willebrand Disease Testing
• Von Willebrand Disease Profile
• 23-Year-Old Female
• Indication for VWF multimer analysis
• Indication for VWF multimer analysis
• Objectives
• Plasma VWF Multimer Analysis
• Example of von Willebrand Factor Multimer Gel Plate
• Solidfied Gel Template
• Gel Template Plasma Sample Wells
• Electrophoresis Separated von Willebrand Factor Multimers
• Plasma VWF Multimer Agarose Slab Gel Electrophoresis — Schema
• VWF Multimer Imaging: Autoradiography vs Infrared In-gel Immunostaining
• VWF Multimer Analysis: Mayo Autoradiograpy — Intermediate-Resolution Gel
• Scanning System for Gels
• VWF Multimer Imaging — Near Infrared In-gel Immunostaining
• Autoradiography and Infrared Method Comparison
• VWF Multimer Analysis
• Objectives
• 23-Year-Old Female: Type 1 VWD
• Type 3 VWD
• Type 2A VWD
• Type 2B VWD
• Type 2M VWD
• VWF Multimers Analysis: Summary
• VWF Multimers Analysis: Summary (2)
• References
• Questions?